Levi is identified as the son of James when James sold to Levi a 50 acre tract of land called "rattlesnake land" for £25. Washington County Deed Book G, p147, Registered 2 May 1831.

Levi was the Executor of the estate of Myles Spruill, his father-in-law. [Deed Book F, page 200, 6 Jun 1824]
1824 Alexander Davenport and _____ Davenport to Aaron Spruill and Selah Spruill, Executor/Executrix
COPIED of Henry Spruill, dec'd and Levi Ambrose, Executor of
Myles Spruill, dec'd . . 400 lbs. 37 acres . .
6 June 1824 Wit: Joshua B. Davis, Reading M. Spruill F 250 1822 Jesse Dillen to Dempsey Spruill

Levi Ambrose 2400020000000-0010010000000

Enumerated 11 Sep 1850 HN322FN322
Levi Ambrose 60 M Farmer 5000 NC
Polly 56 F NC
Brittain 28 M Farmer NC
Levi W. 23 M Farmer NC
Woodsone 19 M Farmer NC
Wilson 15 M NC
Anna 4 F NC

• Married:
• Note:

  F 475 1829 Anson A. Spruill to Daniel Woodley $5 adjoining the
  land which the late Myles Spruill in his will and
  testament bequeathed to my sister Polly, now the wife
  of Levi Ambrose 15 Jan. 1829 Reg. 16 Feb. 1829
  Wit: Baley Woodley
  

1. Britton Buckner AMBROSE b: ABT 1821 in Washington County, North Carolina
2. Benjamin F. AMBROSE b: ABT 1822 in Washington County, North Carolina
3. Francis A. "Frank" AMBROSE b: ABT 1823 in Plymouth, Lees Mills Twp., Washington County, North Carolina
4. Henry AMBROSE b: ABT 1826 in Washington County, North Carolina
5. Levi Warren AMBROSE b: 25 OCT 1827 in Washington County, North Carolina
6. Woodson S. AMBROSE b: OCT 1832 in Washington County, North Carolina
7. Wilson AMBROSE b: 18 MAY 1835 in Washington County, North Carolina

1. Title: 1850 Federal Census
2. Title: U.S. Federal Census (Provo, UT: Ancestry.com [images online],)
   Page: M33_80, p409
3. Title: U.S. Federal Census (Provo, UT: Ancestry.com [images online],)
   Page: M19_25, p559
4. Title: U.S. Federal Census (Provo, UT: Ancestry.com [images online],)
   Page: M704_373 p17
5. Title: U.S. Federal Census (Provo, UT: Ancestry.com [images online],)
   Page: 1850NCM432_648-85